Biological Sciences - Plant Biology Leghemoglobin green derivatives with nitrated hemes evidence production of highly reactive nitrogen species during aging of legume nodules
نویسندگان
چکیده
Departamento de Nutrición Vegetal, Estación Experimental de Aula Dei, Consejo Superior de Investigaciones Científicas (CSIC), Apartado 13034, 50080 Zaragoza, Spain; CSICUAB Proteomics Laboratory, Instituto de Investigaciones Biomédicas de Barcelona-CSIC, Bellaterra, Spain; Universidad de Vigo, Campus As Lagoas, 36200 Vigo, Pontevedra, Spain; Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721-0041; and iBiTec-S / URA 2096 / CEA-Saclay, 91191 Gif-sur-Yvette cedex, France
منابع مشابه
Leghemoglobin green derivatives with nitrated hemes evidence production of highly reactive nitrogen species during aging of legume nodules.
Globins constitute a superfamily of proteins widespread in all kingdoms of life, where they fulfill multiple functions, such as efficient O(2) transport and modulation of nitric oxide bioactivity. In plants, the most abundant Hbs are the symbiotic leghemoglobins (Lbs) that scavenge O(2) and facilitate its diffusion to the N(2)-fixing bacteroids in nodules. The biosynthesis of Lbs during nodule ...
متن کاملLeghemoglobin is nitrated in functional legume nodules in a tyrosine residue within the heme cavity by a nitrite/peroxide-dependent mechanism.
Protein tyrosine (Tyr) nitration is a post-translational modification yielding 3-nitrotyrosine (NO2 -Tyr). Formation of NO2 -Tyr is generally considered as a marker of nitro-oxidative stress and is involved in some human pathophysiological disorders, but has been poorly studied in plants. Leghemoglobin (Lb) is an abundant hemeprotein of legume nodules that plays an essential role as an O2 trans...
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Rhizobia elicit nitrogen-fixing nodules on legume roots composed of highly differentiated plant and bacterial cells. Sustaining symbiosis is energy intensive, and an increase in plant and bacterial heme protein synthesis is an essential feature of nod-ule ontogeny. Symbiotic bacteroids express cytochrome heme proteins as part of an electron transport system that allows vigorous and efficient re...
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Hemoglobins are ubiquitous in nature and among the best-characterized proteins. Genetics has revealed crucial roles for human hemoglobins, but similar data are lacking for plants. Plants contain symbiotic and nonsymbiotic hemoglobins; the former are thought to be important for symbiotic nitrogen fixation (SNF). In legumes, SNF occurs in specialized organs, called nodules, which contain millions...
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Parasponia is the first non-legume genus proven to form nitrogen-fixing root nodules induced by rhizobia. Infiltration with India ink demonstrated that intercellular air spaces are lacking in the inner layers of the nodule cortex. Oxygen must diffuse through these layers to reach the cells containing the rhizobia, and it was calculated that most of the gradient in O(2) partial pressure between ...
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